Molecular genetic surveillance based on the identification of borrelia in ixodid ticks

Aim . To improve emergency diagnostic measures and preventive antimicrobial therapy in patients with ixodid tick bites using molecular genetic techniques for identification of Вorrelia spp. Materials Methods Borrelia strains were isolated by inoculation suspension on the BSK-H medium. Molecular screening was performed real-time polymerase chain reaction (PCR) sequencing. In total, we examined 4,276 including 272 bitten Ixodes persulcatus 3,108 Dermacentor reticulatus. addition, investigated blood 896 signatures Results detected PCR 30.1% 3.2% reticulatus suspensions. However, detection rare, reaching only 1.1% those two cases bites. Genotyping 8 suspensions showed presence garinii 1 sample, afzelii 5 samples, bavariensis miyamotoi sample. Conclusion significant proportion Ixodid ticks bearing spp., indicating need specific therapy.